aav9 gfp u6 scrmb shrna  (Vector Biolabs)

Journal: Science Advances

Article Title: Depolarizing GABA A current in the prefrontal cortex is linked with cognitive impairment in a mouse model relevant for schizophrenia

doi: 10.1126/sciadv.aba5032

Figure Lengend Snippet: Adeno-associated viral constructs containing either mouse Slc12a2-shRNA [short hairpin RNA; knockdown (KD)] or a scrambled RNA sequence (SCR) were injected into the ILC. ( A ) Microphotograph (2×) of the ILC of an injected mouse; green fluorescent protein (GFP) expression identifies the injection sites. ( B ) Map indicating the injection sites for every mouse treated. Black circles identify vehicle/KD mice, blue squares identify scPCP/SCR, and green circles indicate scPCP + KD. ( C ) Results of the NOR test. The sample size was five, six, and five mice, respectively. P = 0.0293 for vehicle/KD versus scPCP/SCR and P = 0.0039 for scPCP/KD versus scPCP/SCR, one-way ANOVA followed by Bonferroni test ( F = 8.16). ( D ) Y-maze spontaneous alternation test. Same mice as in (C). P = 0.0001 for vehicle/KD versus scPCP/SCR and P < 0.0001 for scPCP/KD versus scPCP/SCR ( F = 28.14).

Article Snippet: Adeno-associated virus constructs containing either mouse Slc12a2-shRNA [AAV9-GFP-U6-mSlc12a2-shRNA; titer of stock, 1.6 × 10 13 genome copies (GC)/ml] or scrambled-shRNA (AAV9-GFP-U6-scrmb-shRNA; titer of stock, 3.1 × 10 13 GC/ml) were produced by Vector Biolab (Malvern, PA, USA) and kept at −80°C until used.

Techniques: Construct, shRNA, Sequencing, Injection, Expressing